CD107a / LAMP1 Monoklonaler Antikörper

CD107a / LAMP1 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1D4B

Kat-Nr. : 65050-1-Ig

Synonyme

CD107a, Lamp1, 1D4B, Lamp 1

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  IF Staining of NIH/3T3 using 65050-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 65050-1-Ig (CD107a antibody) at dilution of 1:100 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  FC experiment of NIH/3T3 using 65050-1-Ig

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107a / LAMP1 (65050-1-Ig, Clone:1D4B) and FITC anti-Rat IgG2a antibody at dilution 1:100, or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a antibody at dilution 1:100. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 6 publications below

Produktinformation

65050-1-Ig bindet in IF/ICC, FC (Intra) CD107a / LAMP1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Plasmamembranfraktion einer Mausembryo-NIH/3T3-Zelllinie
• Vollständiger Name: lysosomal-associated membrane protein 1
• GenBank-Zugangsnummer: BC006785
• Gene symbol: CD107a
• Gene ID (NCBI): 16783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Publikationen

Species	Application	Title
mouse	IF	J Cell Physiol; Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes; Authors - Rui Hua
mouse	IF	Cell Struct Funct; Macropinocytosis regulates cytokine expression through Erk signaling in LPS-stimulated macrophages; Authors - Li Wang
mouse	IF	Res Sq; Pathological characteristics of axons and proteome patterns in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency; Authors - Weidong Le
mouse	IF	J Leukoc Biol; Macropinocytic cups function as signal platforms for the mTORC2-AKT pathway to modulate LPS-induced cytokine expression in macrophages; Authors - Li Wang
mouse	IF	Mol Neurodegener; Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency; Authors - Panpan Wang
mouse	IF	J Adv Res; Parkin modulates the hepatocellular carcinoma microenvironment by regulating PD-1/PD-L1 signalling; Authors - Guiqin Ye