CD107a / LAMP1 Monoklonaler Antikörper

CD107a / LAMP1 Monoklonal Antikörper für WB, IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF/ICC, FC (Intra)

Konjugation

Unkonjugiert

Publikationen(11)

CloneNo.

H4A3

Rezensionen (2)

Kat-Nr. : 65051-1-Ig

Synonyme

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3

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• 

  WB analysis using 65051-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 65051-1-Ig (CD107a / LAMP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 65051-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 65051-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 65051-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a / LAMP1 (65051-1-Ig, Clone:H4A3) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 8 publications below

Produktinformation

65051-1-Ig bindet in WB, IF/ICC, FC (Intra) CD107a / LAMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane adhärente periphere Blutzellen
• Vollständiger Name: lysosomal-associated membrane protein 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC006345
• Gene symbol: LAMP1
• Gene ID (NCBI): 3916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Publikationen

Species	Application	Title
human,monkey	WB,IF	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan
human	IF	Eur J Pharmacol; Cycloheptylprodigiosin from marine bacterium Spartinivicinus ruber MCCC 1K03745T induces a novel form of cell death characterized by Golgi disruption and enhanced secretion of cathepsin D in Non-small cell lung cancer cell lines; Authors - Xiaosi Lin
human	IF	Autophagy; ATP6V1D drives hepatocellular carcinoma stemness and progression via both lysosome acidification-dependent and -independent mechanisms; Authors - Zhijie Xu
human	IF	Cell Rep; A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma; Authors - Hongli Zhang
human	IF	MAbs; Targeted protein degradation through site-specific antibody conjugation with mannose 6-phosphate glycan; Authors - Kaori Mukai
mouse	IF	CNS Neurosci Ther; Inhibition of Salt-Inducible Kinase 2 Protects Motor Neurons From Degeneration in ALS by Activating Autophagic Flux and Enhancing mTORC1 Activity; Authors - Weiwei Liang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (09-23-2025)

Works well for IF. Typical lysosomal pattern was observed.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mammalian Cells

FH

Christine (Verified Customer) (10-02-2024)

I randomly tried this antibody for western blotting (because this clone has been shown to work in WB in publications) and it worked beautifully at 1:500 on total lysates of HEK293T cells. Could be diluted much further.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T