CD107a / LAMP1 Monoklonaler Antikörper

CD107a / LAMP1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

3B2E9

Kat-Nr. : 67300-1-Ig

Synonyme

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker

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Galerie der Validierungsdaten

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  WB analysis using 67300-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HeLa using 67300-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67300-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67300-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67300-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67300-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 67300-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD107a antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 67300-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD107a antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HeLa-Zellen, HepG2-Zellen, JAR-Zellen, Jurkat-Zellen, K-562-Zellen, MDA-MB-231-Zellen, THP-1-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 4 publications below

Produktinformation

67300-1-Ig bindet in WB, IHC, IF, ELISA CD107a / LAMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD107a / LAMP1 fusion protein Ag28869
• Vollständiger Name: lysosomal-associated membrane protein 1
• Berechnetes Molekulargewicht: 417 aa, 45 kDa
• Beobachtetes Molekulargewicht: 90-120 kDa
• GenBank-Zugangsnummer: BC021288
• Gene symbol: LAMP1
• Gene ID (NCBI): 3916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Publikationen

Species	Application	Title
human	IF	Int J Mol Sci; Generation and Characterization of SORT1-Targeted Antibody-Drug Conjugate for the Treatment of SORT1-Positive Breast Tumor; Authors - Weiliang Zhuang
human	WB,IF	Cancers (Basel); A Novel Phenazine Analog, CPUL1, Suppresses Autophagic Flux and Proliferation in Hepatocellular Carcinoma: Insight from Integrated Transcriptomic and Metabolomic Analysis; Authors - Jiaqin Chen
mouse	WB	MedComm (2020); Leucine-rich repeat kinase 2 (LRRK2) inhibition upregulates microtubule-associated protein 1B to ameliorate lysosomal dysfunction and parkinsonism; Authors - Kang Chen
	IF	J Nanobiotechnology; A novel "prime and pull" strategy mediated by the combination of two dendritic cell-targeting designs induced protective lung tissue-resident memory T cells against H1N1 influenza virus challenge; Authors - Zhannan Wang
mouse	WB	Antioxidants (Basel); Stimulation of Hepatic Ferritinophagy Mitigates Irp2 Depletion-Induced Anemia; Authors - Yutong Liu
	IF	Nat Commun; Cholesterol removal improves performance of a model biomimetic system to co-deliver a photothermal agent and a STING agonist for cancer immunotherapy; Authors - Lin Li