CD11a/Integrin Alpha L Monoklonaler Antikörper

CD11a/Integrin Alpha L Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1E3H12

Kat-Nr. : 66256-1-Ig

Synonyme

CD11a, 1E3H12, CD11 antigen-like family member A, Integrin alpha L, Integrin alpha-L

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  WB analysis of Ramos using 66256-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66256-1-Ig (CD11a/Integrin Alpha L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66256-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66256-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66256-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66256-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of Jurkat using 66256-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66256-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA CD11a/Integrin Alpha L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD11a/Integrin Alpha L fusion protein Ag7813
• Vollständiger Name: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Berechnetes Molekulargewicht: 129 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC008777
• Gene symbol: CD11a
• Gene ID (NCBI): 3683
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

Publikationen

Species	Application	Title
human	WB	Clin Cosmet Investig Dermatol; Identification of a Gene Expression Signature to Predict the Risk of Abdominal Aortic Aneurysm in Psoriasis Patients; Authors - Xintong Lyu