CD146/MCAM Monoklonaler Antikörper

CD146/MCAM Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, Hund, Kaninchen, Maus

Anwendung

WB, IF/ICC, FC

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

P1H12

Kat-Nr. : 65181-1-Ig

Synonyme

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

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  IF Staining of HUVEC using 65181-1-Ig

  Immunofluorescent analysis of un-fixed HUVEC cells using CD146 antibody (65181-1-Ig, Clone: P1H12 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

• 

  FC experiment of HeLa using 65181-1-Ig

  1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human CD146 (65181-1-Ig, Clone:P1H12) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below
• FC: See 3 publications below

Produktinformation

65181-1-Ig bindet in WB, IF/ICC, FC CD146/MCAM und zeigt Reaktivität mit human, Hund, Kaninchen, Maus

• Getestete Reaktivität: human, Hund, Kaninchen, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gezüchtete humane Nabelschnurzellen
• Vollständiger Name: melanoma cell adhesion molecule
• Berechnetes Molekulargewicht: 646 aa, 72 kDa
• GenBank-Zugangsnummer: BC056418
• Gene symbol: CD146
• Gene ID (NCBI): 4162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Mit Protein-A-Affinitätschromatographie gereinigt
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Publikationen

Species	Application	Title
human	FC	Front Cell Dev Biol; Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.; Authors - Xun Xi
human	FC	Stem Cell Res Ther; Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models; Authors - Han Li
human	IF	J Mol Histol; Effects of simulated microgravity on dental pulp stem cell stemness; Authors - Huailong Hou
human	WB	Front Pharmacol; CD146 promotes resistance of NSCLC brain metastases to pemetrexed via the NF-κB signaling pathway; Authors - Hao Qu
human	FC	Biochim Biophys Acta Mol Cell Res; Senolytic elimination of senescent cells improved periodontal ligament stem cell-based bone regeneration partially through inhibiting YAP; Authors - Linglu Jia