CD146/MCAM Monoklonaler Antikörper

CD146/MCAM Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, Hund, Kaninchen, Maus

Anwendung

WB, IF/ICC, FC

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

P1H12

Kat-Nr. : 65181-1-Ig

Synonyme

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18



Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHUVEC-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:500-1:2000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65181-1-Ig bindet in WB, IF/ICC, FC CD146/MCAM und zeigt Reaktivität mit human, Hund, Kaninchen, Maus

Getestete Reaktivität human, Hund, Kaninchen, Maus
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Gezüchtete humane Nabelschnurzellen
Vollständiger Name melanoma cell adhesion molecule
Berechnetes Molekulargewicht 646 aa, 72 kDa
GenBank-ZugangsnummerBC056418
Gene symbol CD146
Gene ID (NCBI) 4162
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Mit Protein-A-Affinitätschromatographie gereinigt
Lagerungspuffer PBS with 0.09% sodium azide
LagerungsbedingungenStore at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CD146/MCAM antibody 65181-1-IgProtokoll herunterladen
FC protocol for CD146/MCAM antibody 65181-1-IgDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanFC

Front Cell Dev Biol

Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.

Authors - Xun Xi
humanFC

Stem Cell Res Ther

Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models

Authors - Han Li
humanIF

J Mol Histol

Effects of simulated microgravity on dental pulp stem cell stemness

Authors - Huailong Hou
humanWB

Front Pharmacol

CD146 promotes resistance of NSCLC brain metastases to pemetrexed via the NF-κB signaling pathway

Authors - Hao Qu
humanFC

Biochim Biophys Acta Mol Cell Res

Senolytic elimination of senescent cells improved periodontal ligament stem cell-based bone regeneration partially through inhibiting YAP

Authors - Linglu Jia