CD147 Monoklonaler Antikörper

CD147 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1G12B5

Kat-Nr. : CL594-66443

Synonyme

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF

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Galerie der Validierungsdaten

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  IF Staining of human tonsillitis using CL594-66443

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD147 antibody (CL594-66443, Clone: 1G12B5 ) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-66443

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD147 antibody (CL594-66443, Clone: 1G12B5 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL594-66443

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CD147 (CL594-66443, Clone:1G12B5) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66443 bindet in IF, FC (Intra) CD147 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD147 fusion protein Ag2619
• Vollständiger Name: basigin (Ok blood group)
• Berechnetes Molekulargewicht: 385 aa, 42 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC009040
• Gene symbol: BSG
• Gene ID (NCBI): 682
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BSG, also named as 5F7, Basigin, EMMPRIN, TCSF and CD147, plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. It seems to be a receptor for oligomannosidic glycans. CD147 is a receptor of CypA, inducing matrix metalloproteinase expression and mediating the degradation of the extracellular matrix, plays an important role in tumorigenesis and invasion in oral cancer. It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C