CD16/CD32 Monoklonaler Antikörper

CD16/CD32 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2.4G2

Kat-Nr. : 65080-1-Ig

Synonyme

CD32, 2.4G2, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB

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  FC experiment of mouse splenocytes using 65080-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD16 / CD32 (2.4G2) (65080-1-Ig, Clone: 2.4G2) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2) and FITC anti-rat IgG2b Antibody. Cells were co-stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

65080-1-Ig bindet in FC CD16/CD32 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Fc receptor, IgG, low affinity IIb
• GenBank-Zugangsnummer: BC038070
• Gene symbol: CD16/32
• Gene ID (NCBI): 14130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898). Rat anti mouse CD16/CD32 antibody, clone 2.4G2 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.

Publikationen

Species	Application	Title
	FC	Breast Cancer Res Treat; Enhanced antitumor efficacy of bispecific antibody blocking PD-L1 and LAG-3 with doxorubicin: mechanism and safety evaluation; Authors - Chenxing Zhang
mouse	FC	Front Pharmacol; In-situ formed thermosensitive hydrogel amplifies statin-mediated immune checkpoint blockade for coordinated tumor chemo-immunotherapy; Authors - Zefan Liu