CD163 Monoklonaler Antikörper
CD163 Monoklonal Antikörper für FC
Wirt / Isotyp
Maus / IgG1, kappa
Getestete Reaktivität
human
Anwendung
IF, FC
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
GHI/61
Kat-Nr. : CL488-65169
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in FC | humane PBMCs | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| IF | See 1 publications below | 
| FC | See 2 publications below | 
Produktinformation
CL488-65169 bindet in IF, FC CD163 und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| In Publikationen genannte Reaktivität | human | 
| Wirt / Isotyp | Maus / IgG1, kappa | 
| Klonalität | Monoklonal | 
| Typ | Antikörper | 
| Immunogen | Glykoprotein-Zubereitung aus Haarzell-Leukämie-Milz | 
| Vollständiger Name | CD163 molecule | 
| Berechnetes Molekulargewicht | 1156 aa, 125 kDa | 
| GenBank-Zugangsnummer | BC051281 | 
| Gene symbol | CD163 | 
| Gene ID (NCBI) | 9332 | 
| Konjugation | CoraLite® Plus 488 Fluorescent Dye | 
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm | 
| Form | Liquid | 
| Reinigungsmethode | Protein-G-Reinigung | 
| Lagerungspuffer | PBS with 0.09% sodium azide and 0.5% BSA | 
| Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. | 
Hintergrundinformationen
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| FC protocol for CL Plus 488 CD163 antibody CL488-65169 | Download protocol | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Oncol Lett Tumor-associated macrophage-derived exosomes promote EGFR-TKI resistance in non-small cell lung cancer by regulating the AKT, ERK1/2 and STAT3 signaling pathways | ||
| Cell Mol Life Sci Tumor-associated macrophage-derived exosomal miR21-5p promotes tumor angiogenesis by regulating YAP1/HIF-1α axis in head and neck squamous cell carcinoma | ||
| Cell Death Dis Tumor-derived exosomal CCT6A serves as a matchmaker introducing chemokines to tumor-associated macrophages in pancreatic ductal adenocarcinoma | 

