CD163 Monoklonaler Antikörper

CD163 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(3)

CloneNo.

GHI/61

Kat-Nr. : CL488-65169

Synonyme

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

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  FC experiment of human PBMCs using CL488-65169

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD163 (CL488-65169, Clone:GHI/61) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-65124). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using CL488-65169

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD163 (CL488-65169, Clone:GHI/61) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-65124). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 2 publications below

Produktinformation

CL488-65169 bindet in IF, FC CD163 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Glykoprotein-Zubereitung aus Haarzell-Leukämie-Milz
• Vollständiger Name: CD163 molecule
• Berechnetes Molekulargewicht: 1156 aa, 125 kDa
• GenBank-Zugangsnummer: BC051281
• Gene symbol: CD163
• Gene ID (NCBI): 9332
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Publikationen

Species	Application	Title
human	FC	Oncol Lett; Tumor-associated macrophage-derived exosomes promote EGFR-TKI resistance in non-small cell lung cancer by regulating the AKT, ERK1/2 and STAT3 signaling pathways; Authors - Shiyang Yuan
human	FC	Cell Mol Life Sci; Tumor-associated macrophage-derived exosomal miR21-5p promotes tumor angiogenesis by regulating YAP1/HIF-1α axis in head and neck squamous cell carcinoma; Authors - Quan Yan
human	IF	Cell Death Dis; Tumor-derived exosomal CCT6A serves as a matchmaker introducing chemokines to tumor-associated macrophages in pancreatic ductal adenocarcinoma; Authors - Tianyin Ma