CD20 Monoklonaler Antikörper

CD20 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF-P

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4A7G3

Kat-Nr. : CL647-60271

Synonyme

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

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  IF Staining of human lung cancer using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite®555 pan-keratin antibody (CL555-26411, orange).

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  IF Staining of human lung cancer using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite®555 pan-keratin antibody (CL555-26411, orange).

• 

  IF Staining of human lung cancer using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®488 pan-keratin antibody (CL488-26411, green).

• 

  IF Staining of human tonsillitis using CL647-60271

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL647-60271 (CD20 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Lungenkarzinomgewebe, humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-60271 bindet in IF-P CD20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD20 fusion protein Ag17309
• Vollständiger Name: membrane-spanning 4-domains, subfamily A, member 1
• Berechnetes Molekulargewicht: 297 aa, 33 kDa
• GenBank-Zugangsnummer: BC002807
• Gene symbol: CD20
• Gene ID (NCBI): 931
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.