CD22 Monoklonaler Antikörper

CD22 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

7A2F1

Rezensionen (1)

Kat-Nr. : 66103-1-Ig

Synonyme

7A2F1, B cell receptor CD22, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule

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  WB analysis of Ramos using 66103-1-Ig

  Ramos, Daudi, and Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66103-1-Ig

  Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  IHC staining of human tonsillitis using 66103-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66103-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66103-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66103-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66103-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66103-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66103-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of Raji using 66103-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ramos-Zellen, Daudi-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Appendizitis-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2500-1:10000
• Immunhistochemie (IHC): IHC : 1:10000-1:40000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

66103-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA CD22 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD22 fusion protein Ag17986
• Vollständiger Name: CD22 molecule
• Berechnetes Molekulargewicht: 847 aa, 95 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC109306
• Gene symbol: CD22
• Gene ID (NCBI): 933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

Publikationen

Species	Application	Title
human	IF	J Biomed Res; Causal genetic regulation of DNA replication on immune microenvironment in colorectal tumorigenesis: evidenced by an integrated approach of trans-omics and GWAS; Authors - Sumeng Wang
	WB	Free Radic Biol Med; Extracellular vesicles released by ALL patients contain HNE-adducted proteins: Implications of collateral damage; Authors - Jenni Ho

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Prostate

CD22 Antibody Immunofluorescence validation (1:100 dilution) in Prostate (Cat no:66103-1-Ig)