TEM1 Polyklonaler Antikörper

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• KD/KO Validated

TEM1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 18160-1-AP

Synonyme

CD248, CD164L1, CD248 molecule, endosialin, Endosialin, TEM-1

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  WB analysis of HUVEC using 18160-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 18160-1-AP (TEM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18160-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18160-1-AP (TEM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 18160-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18160-1-AP (TEM1 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 18160-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18160-1-AP (TEM1 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18160-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18160-1-AP (TEM1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18160-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18160-1-AP (TEM1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18160-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18160-1-AP (TEM1 antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human lung cancer using 18160-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18160-1-AP (TEM1 antibody) at dilution of 1:500 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 10 publications below
• CoIP: See 1 publications below

Produktinformation

18160-1-AP bindet in WB, IHC, IF, CoIP, ELISA TEM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TEM1 fusion protein Ag12990
• Vollständiger Name: CD248 molecule, endosialin
• Berechnetes Molekulargewicht: 757 aa, 81 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC051340
• Gene symbol: TEM1
• Gene ID (NCBI): 57124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.

Publikationen

Species	Application	Title
human	IF	Cancer Cell; Cancer-associated fibroblast phenotypes are associated with patient outcome in non-small cell lung cancer; Authors - Lena Cords
human	IF	Cancer Res; Interaction with CD68 and regulation of GAS6 expression by endosialin in fibroblasts drives recruitment and polarization of macrophages in hepatocellular carcinoma.; Authors - Fa Yang
rat	CoIP	Clin Transl Med; CD248 as a novel therapeutic target in pulmonary arterial hypertension.; Authors - Tao Xu
mouse,human	WB,IHC	J Invest Dermatol; Tumor Endothelial Marker 1 (TEM1/Endosialin/CD248) Enhances Wound Healing by Interacting with Platelet-Derived Growth Factor Receptors.; Authors - Yi-Kai Hong
mouse	IF	EBioMedicine; Specific loss of adipocyte CD248 improves metabolic health via reduced white adipose tissue hypoxia, fibrosis and inflammation.; Authors - Paul Petrus
human	WB,IHC	Oncotarget; Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma.; Authors - Emily Capone