CD25/IL-2RA Monoklonaler Antikörper

CD25/IL-2RA Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG1, lambda

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

PC61.5

Kat-Nr. : CL647-65137

Synonyme

CD25, Il-2ra, Il2ra, IL-2R subunit alpha, Il2r

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  FC experiment of mouse splenocytes using CL647-65137

  1X10^6 mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD25 (CL647-65137, Clone: PC61.5) or CoraLite® Plus 647-Conjugated rat IgG2a isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65137 bindet in FC CD25/IL-2RA und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: interleukin 2 receptor, alpha chain
• GenBank-Zugangsnummer: BC114437
• Gene symbol: CD25
• Gene ID (NCBI): 16184
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).