CD3 Monoklonaler Antikörper

CD3 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

IHC, FC, Cell treatment

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

OKT3

Kat-Nr. : 65133-1-Ig

Synonyme

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, OKT3

---

• 

  FC experiment of human PBMCs using 65133-1-Ig

  1X10^6 human PBMCs were surface co-stained with FITC Anti-human CD19 and 0.2 ug Anti-Human CD3 (65133-1-Ig, Clone: OKT3) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG2a (KFA043) or CoraLite® Plus 647 conjugated Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65133-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65133-1-Ig, Clone: OKT3) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG2a (KFA043) (red) or CoraLite® Plus 647 conjugated Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

65133-1-Ig bindet in IHC, FC, Cell treatment CD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human T cells
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3
• Gene ID (NCBI): 916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publikationen

Species	Application	Title
mouse	IHC	Front Immunol; Efficacy and safety evaluation of cross-reactive Fibroblast activation protein scFv-based CAR-T cells; Authors - Wenhao Niu
human	Cell treatment	J Pharm Anal; Secondary metabolites of mulberry leaves exert anti-lung cancer activity through regulating the PD-L1/PD-1 signaling pathway; Authors - Guiqin Ye
		Signal Transduct Target Ther; N-deglycosylation targeting chimera (DGlyTAC): a strategy for immune checkpoint proteins inactivation by specifically removing N-glycan; Authors - Li Li