CD3 Rekombinanter Antikörper

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• KD/KO Validated

CD3 Rekombinant Antikörper für IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4I19

Kat-Nr. : CL488-81324

Synonyme

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

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  IF Staining of mouse spleen using CL488-81324

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite® Plus 488 CD3 antibody (CL488-81324, Clone: 4I19 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using CL488-81324

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD3 (CL488-81324, Clone:4I19) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Mausmilzgewebe
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-81324 bindet in IF-P CD3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD3 fusion protein Ag11797
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23-25 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3
• Gene ID (NCBI): 916
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.