CD3 Monoklonaler Antikörper

CD3 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3F3A1

Kat-Nr. : CL647-60181

Synonyme

CD3E, T3E, TCRE

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Galerie der Validierungsdaten

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  IF Staining of human colon cancer using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

• 

  IF Staining of human colon cancer using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Cyan) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

• 

  IF Staining of human colon cancer using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:200, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, red), CoraLite® Plus 488 pan-keratin antibody (CL488-26411, green).

• 

  IF Staining of human tonsillitis using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:100, CoraLite®488 CD163 antibody (CL488-16646, green), CoraLite®555 Beta Catenin antibody (CL555-51067, orange).

• 

  IF Staining of human tonsillitis using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-60181 bindet in IF CD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD3 fusion protein Ag11797
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3E
• Gene ID (NCBI): 916
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.