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CD300LF Polyklonaler Antikörper

CD300LF Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13334-1-AP

Synonyme

CD300f, CD300LF, CLM 1, CLM1, CMRF35 like molecule 1, IgSF13, IREM 1, IREM1, NK inhibitory receptor, NKIR

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using CD300LF Polyclonal antibody (13334-1-AP)

    WB analysis using 13334-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13334-1-AP (CD300LF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD300LF Polyclonal antibody (13334-1-AP)

    WB analysis of U-937 using 13334-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 13334-1-AP (CD300LF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using CD300LF Polyclonal antibody (13334-1-AP)

    IP experiment of K-562 using 13334-1-AP

    IP Result of anti-CD300LF (IP:13334-1-AP, 4ug; Detection:13334-1-AP 1:1500) with K-562 cells lysate 4000ug.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen, U-937-Zellen
Erfolgreiche IPK-562-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

13334-1-AP bindet in WB, IP, ELISA CD300LF und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte ReaktivitätMaus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD300LF fusion protein Ag4150
Vollständiger Name CD300 molecule-like family member f
Berechnetes Molekulargewicht 290 aa, 32 kDa
Beobachtetes Molekulargewicht 30-34 kDa
GenBank-ZugangsnummerBC028199
Gene symbol CD300LF
Gene ID (NCBI) 146722
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD300LF, also known as CLM1 (CMRF35-like molecule 1), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LF is an inhibitory receptor that has been shown to inhibit various inflammatory processes in myeloid cells, such as macrophages and mast cells.

Protokolle

Produktspezifische Protokolle
WB protocol for CD300LF antibody 13334-1-APProtokoll herunterladen
IP protocol for CD300LF antibody 13334-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

BMC Pulm Med

Identification of new biomarkers for Acute Respiratory Distress Syndrome by expression-based genome-wide association study.

Authors - Dmitry N Grigoryev
View Article