CD300LF Polyklonaler Antikörper

CD300LF Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 13334-1-AP

Synonyme

CD300 antigen-like family member F, CD300f, CLM 1, CLM1, CLM-1

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  WB analysis using 13334-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13334-1-AP (CD300LF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 13334-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13334-1-AP (CD300LF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13334-1-AP

  IP result of anti-CD300LF (IP:13334-1-AP, 4ug; Detection:13334-1-AP 1:1500) with K-562 cells lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, U-937-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

13334-1-AP bindet in WB, IF, IP, ELISA CD300LF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD300LF fusion protein Ag4150
• Vollständiger Name: CD300 molecule-like family member f
• Berechnetes Molekulargewicht: 290 aa, 32 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC028199
• Gene symbol: CD300LF
• Gene ID (NCBI): 146722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD300LF, also known as CLM1 (CMRF35-like molecule 1), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LF is an inhibitory receptor that has been shown to inhibit various inflammatory processes in myeloid cells, such as macrophages and mast cells.

Publikationen

Species	Application	Title
	WB	BMC Pulm Med; Identification of new biomarkers for Acute Respiratory Distress Syndrome by expression-based genome-wide association study.; Authors - Dmitry N Grigoryev
human	WB	J Inflamm Res; Quercetin Attenuates MRGPRX2-Mediated Mast Cell Degranulation via the MyD88/IKK/NF-κB and PI3K/AKT/ Rac1/Cdc42 Pathway; Authors - Chenrui Zhao
human	WB,IF	Phytother Res; Quercetin Alleviates Chronic Urticaria by Negatively Regulating IgE-Mediated Mast Cell Activation Through CD300f; Authors - Chenrui Zhao ; KD Validated