CD38 Monoklonaler Antikörper

CD38 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

90

Kat-Nr. : CL647-65059

Synonyme

Cd38, CD38 antigen, Cd38 rs1

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Galerie der Validierungsdaten

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  IF Staining of mouse splenocytes using CL647-65059

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD38 (CL647-65059, Clone: 90) at dilution of 1:400.

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  FC experiment of mouse splenocytes using CL647-65059

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3ε (FITC-65060, Clone: 145-2C11) and CoraLite® Plus 647-conjugated Rat IgG2a isotype control (left) or 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse CD38 (CL647-65059, Clone: 90) (right). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65059 bindet in IF, FC CD38 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-Knochenmark-Vorläufer-B-Zellen
• Vollständiger Name: CD38 antigen
• GenBank-Zugangsnummer: BC046312
• Gene symbol: Cd38
• Gene ID (NCBI): 12494
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).