CD49b Monoklonaler Antikörper

CD49b Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgM, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

DX5

Kat-Nr. : CL488-65065

Synonyme

CD49B, DX5, integrin alpha 2, Itga2

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using CL488-65065

  1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse NK1.1 (CD161) (PK136) (APC-65138, Clone: PK136) and CoraLite® Plus 488-conjugated Rat IgM isotype control antibody (left) or 5.00 ul CoraLite® Plus 488-conjugated Anti-Mouse CD49b (CL488-65065, Clone: DX5) (right). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65065 bindet in FC CD49b und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgM, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Aus C57BL/6-Mäusen isolierte NK-Zellen
• Vollständiger Name: integrin alpha 2
• GenBank-Zugangsnummer: BC065139
• Gene symbol: Itga2
• Gene ID (NCBI): 16398
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.