CD68 Rekombinanter Antikörper

CD68 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

KP1

Kat-Nr. : CL405-65593

Synonyme

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

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  FC experiment of human PBMCs using CL405-65593

  1x10^6 human PBMCs were intracellularly stained with APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC) and 5 ul CoraLite® Plus 405 Anti-Human CD68 (KP1) Mouse IgG2a RecAb (CL405-65593, Clone: KP1) or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using CL405-65593

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD68 (KP1) Mouse IgG2a RecAb (CL405-65593, Clone: KP1) (red) or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL405-65593 bindet in FC (Intra) CD68 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD68 molecule
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC015557
• Gene symbol: CD68
• Gene ID (NCBI): 968
• Konjugation: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.