CD68 Polyklonaler Antikörper

CD68 Polyklonal Antikörper für IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF-P

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(2)

Kat-Nr. : CL594-25747

Synonyme

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

---

• 

  IF Staining of human colon using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200, Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), Collagen Type III (N-terminal) antibody (22734-1-AP, green).

• 

  IF Staining of human tonsillitis using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200.

• 

  IF Staining of human colon using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:50, CoraLite®488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4, green).

• 

  IF Staining of human colon using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:50, CoraLite®488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4, green).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Kolongewebe, humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

CL594-25747 bindet in IF-P CD68 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD68 fusion protein Ag22815
• Vollständiger Name: CD68 molecule
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC015557
• Gene symbol: CD68
• Gene ID (NCBI): 968
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.

Publikationen

Species	Application	Title
mouse	IF	Front Mol Biosci; Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress.; Authors - Peng Zou
mouse	IF	Phytomedicine; Acacetin alleviates rheumatoid arthritis by targeting HSP90 ATPase domain to promote COX-2 degradation; Authors - Wenshuang Wang