CD79a Rekombinanter Antikörper

CD79a Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241299F3

Kat-Nr. : 98072-1-RR

Synonyme

241299F3, B-cell antigen receptor complex-associated protein alpha chain, MB 1, MB 1 membrane glycoprotein, MB1

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  FC experiment of human PBMCs using 98072-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a Rabbit RecAb (98072-1-RR, Clone: 241299F3) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98072-1-RR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a Rabbit RecAb (98072-1-RR, Clone: 241299F3) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

• 

  Affinity and Kinetic Characterization of 98072-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98072-1-RR against Human CD79a were performed. The affinity constant is 0.151 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98072-1-RR bindet in FC (Intra) CD79a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD79a molecule, immunoglobulin-associated alpha
• Berechnetes Molekulargewicht: 226 aa, 25 kDa
• GenBank-Zugangsnummer: BC113733
• Gene symbol: CD79A
• Gene ID (NCBI): 973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.