CD86 Monoklonaler Antikörper

CD86 Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF/ICC, FC

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

BU63

Rezensionen (1)

Kat-Nr. : 65165-1-Ig

Synonyme

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

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  IF Staining of Raji using 65165-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD86 antibody (65165-1-Ig, Clone: BU63 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Daudi using 65165-1-Ig

  1X10^6 Daudi cells were surface stained with 0.20 ug Anti-Human CD86 (65165-1-Ig, Clone: BU63) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Raji-Zellen
• Erfolgreiche Detektion in FC: Daudi-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

65165-1-Ig bindet in IF/ICC, FC CD86 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: B-lymphoblastoide Zelllinie ARH 77
• Vollständiger Name: CD86 molecule
• Berechnetes Molekulargewicht: 329 aa, 38 kDa
• GenBank-Zugangsnummer: BC040261
• Gene symbol: CD86
• Gene ID (NCBI): 942
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Publikationen

Species	Application	Title
human	IF	J Agric Food Chem; Novel Collagen Analogs with Multicopy Mucin-Type Sequences for Multifunctional Enhancement Properties Using SUMO Fusion Tags; Authors - Zilong Zhao
human	FC	Int Immunopharmacol; Isosilybin A exhibits anti-inflammatory properties in rosacea by inhibiting MAPK pathway and M1 macrophage polarization; Authors - Chen-Chen Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paul (Verified Customer) (02-13-2025)

Works well for immunofluorescence. Clear signal in high expressing B cell line at 1/400 dilution.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: B cell lymphoma