CD8a Monoklonaler Antikörper

CD8a Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, FC

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

RPA-T8

Kat-Nr. : 65144-1-Ig

Synonyme

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

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Galerie der Validierungsdaten

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  FC experiment of human peripheral blood lymphocytes using 65144-1-Ig

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD8a (65144-1-Ig, Clone:RPA-T8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

65144-1-Ig bindet in WB, IHC, FC CD8a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD8a molecule
• Berechnetes Molekulargewicht: 235 aa, 26 kDa
• GenBank-Zugangsnummer: BC025715
• Gene symbol: CD8A
• Gene ID (NCBI): 925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid, pH 7,2.
• Lagerungsbedingungen: Bei 2-8℃ lagern.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor Immunity.; Authors - Ling Ding
human	IHC	Front Oncol; Comprehensive Characterization of Immune Landscape Based on Epithelial-Mesenchymal Transition Signature in OSCC: Implication for Prognosis and Immunotherapy.; Authors - Si-Yuan Zhang
human	IHC	Front Oncol; Typing and modeling of hepatocellular carcinoma based on disulfidptosis-related amino acid metabolism genes for predicting prognosis and guiding individualized treatment; Authors - Xuenuo Chen
pig	IHC	J Virol; Combinational Deletions of MGF360-9L and MGF505-7R Attenuated Highly Virulent African Swine Fever Virus and Conferred Protection against Homologous Challenge.; Authors - Mingyang Ding