CD8a Monoklonaler Antikörper

CD8a Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

RPA-T8

Kat-Nr. : 65144-1-Ig

Synonyme

CD8, RPA-T8, p32, MAL, Leu2

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  FC experiment of human PBMCs using 65144-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD8a (RPA-T8) (65144-1-Ig) or 0.2 ug Mouse IgG1 Isotype Control and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with 5 ul CoraLite® Plus 488 Anti-Human CD3 (CL488-65151). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65144-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD8a (RPA-T8) (65144-1-Ig) or 0.2 ug Mouse IgG1 Isotype Control and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

65144-1-Ig bindet in FC CD8a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD8a molecule
• Berechnetes Molekulargewicht: 235 aa, 26 kDa
• GenBank-Zugangsnummer: BC025715
• Gene symbol: CD8A
• Gene ID (NCBI): 925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor Immunity.; Authors - Ling Ding
human	IHC	Front Oncol; Comprehensive Characterization of Immune Landscape Based on Epithelial-Mesenchymal Transition Signature in OSCC: Implication for Prognosis and Immunotherapy.; Authors - Si-Yuan Zhang
human	IHC	Front Oncol; Typing and modeling of hepatocellular carcinoma based on disulfidptosis-related amino acid metabolism genes for predicting prognosis and guiding individualized treatment; Authors - Xuenuo Chen
		J Virol; Combinational Deletions of MGF360-9L and MGF505-7R Attenuated Highly Virulent African Swine Fever Virus and Conferred Protection against Homologous Challenge.; Authors - Mingyang Ding