CD97 Monoklonaler Antikörper

CD97 Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3F10C2

Kat-Nr. : 66972-1-Ig

Synonyme

CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 66972-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 66972-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of DC2.4 using 66972-1-Ig

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66972-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 66972-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (66972-1-Ig, Clone: 3F10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of Jurkat using 66972-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human CD97 (66972-1-Ig, Clone:3F10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, DC2.4-Zellen, HepG2-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IF: Jurkat-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66972-1-Ig bindet in WB, IF, FC, ELISA CD97 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD97 fusion protein Ag27991
• Vollständiger Name: CD97 molecule
• Berechnetes Molekulargewicht: 722 aa, 79 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC026690
• Gene symbol: CD97
• Gene ID (NCBI): 976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C