Cdc20 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cdc20 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Kat-Nr. : 10252-1-AP

Synonyme

CDC20, CDC20A, p55CDC

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Galerie der Validierungsdaten

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  WB analysis using 10252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10252-1-AP

  WB result of Cdc20 antibody (10252-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cdc20 transfected HeLa cells.

• 

  WB analysis using 10252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10252-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10252-1-AP (CDC20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10252-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10252-1-AP (CDC20 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10252-1-AP

  IP Result of anti-CDC20 (IP:10252-1-AP, 4ug; Detection:10252-1-AP 1:800) with Jurkat cells lysate 3200ug.

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  IHC staining of human breast cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (Cdc20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10252-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10252-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cdc20 antibody (10252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, PC-3-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 25 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

10252-1-AP bindet in WB, IP, IHC, IF, ELISA Cdc20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cdc20 fusion protein Ag0295
• Vollständiger Name: cell division cycle 20 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC001088
• Gene symbol: CDC20
• Gene ID (NCBI): 991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

Publikationen

Species	Application	Title
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Elife; Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit.; Authors - James Holder
human	WB	Int J Mol Sci; Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells.; Authors - Yang Gao ; KD Validated
human	WB	Cell Death Dis; Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma.; Authors - Yanlin Huang
human	WB	Aging (Albany NY); The bioinformatics and experimental analysis of the novel roles of virus infection-associated gene CDC20 for prognosis and immune infiltration in hepatocellular carcinoma.; Authors - Juanni Li

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