CDC25A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CDC25A Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 55031-1-AP

Synonyme

CDC25A, CDC25A2, M phase inducer phosphatase 1

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 55031-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55031-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 55031-1-AP

  IP Result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug.

• 

  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 55031-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human breast cancer using 55031-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

55031-1-AP bindet in WB, IP, IHC, IF, ELISA CDC25A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cell division cycle 25 homolog A (S. pombe)
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_001789
• Gene symbol: CDC25A
• Gene ID (NCBI): 993
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: 0,1 M NaHCO3, 0,1M Glyzin, 0,02% Natriumazid und 50% Glyzerin, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.

Publikationen

Species	Application	Title
human	WB	Nat Commun; The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells.; Authors - Xiao Gao
human	WB	Nat Commun; Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.; Authors - Jiliang Xia
human	WB	Cancer Res; YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.; Authors - Jingnan Pi
human	WB,IHC,IF	Cell Death Differ; Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer.; Authors - Soumyadip Das
mouse	WB	Carbohydr Polym; Sulfate oligosaccharide of Gracilaria lemaneiformis modulates type 1 immunity by restraining T cell activation.; Authors - Qingmei Liu
human	WB	Oncogene; PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.; Authors - Xisheng Yang