CDC27; APC3 Polyklonaler Antikörper

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• KD/KO Validated

CDC27; APC3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 10918-1-AP

Synonyme

CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3

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  WB analysis using 10918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10918-1-AP (CDC27; APC3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10918-1-AP

  IP result of anti-CDC27; APC3 (IP:10918-1-AP, 3ug; Detection:10918-1-AP 1:600) with K-562 cells lysate 11000ug.

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  IHC staining of human prostate cancer using 10918-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10918-1-AP (CDC27; APC3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 10918-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using CDC27 antibody 10918-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Produktinformation

10918-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CDC27; APC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDC27; APC3 fusion protein Ag1345
• Vollständiger Name: cell division cycle 27 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC011656
• Gene symbol: CDC27
• Gene ID (NCBI): 996
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

Publikationen

Species	Application	Title
human	WB	Nat Commun; F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression; Authors - Feng Li
human	WB,IHC	Front Oncol; CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma.; Authors - Lin Qiu ; KD Validated
	WB	J Biol Chem; The ubiquitin E3 ligase APC/CCdc20 mediates mitotic degradation of OGT; Authors - Li Meng
human	IHC	Oncotarget; mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27.; Authors - Zhuoying Feng
human	WB	Cell Death Dis; EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer.; Authors - Yiming He
human	IP	Cell Commun Signal; APC/C-regulated CPT1C promotes tumor progression by upregulating the energy supply and accelerating the G1/S transition; Authors - Huihui Zhao