CDC6 Polyklonaler Antikörper

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• KD/KO Validated

CDC6 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 11640-1-AP

Synonyme

Cdc18 related protein, CDC18L, CDC6, CDC6 related protein, HsCDC18, HsCDC6, p62(cdc6)

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11640-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11640-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11640-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 11640-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11640-1-AP (CDC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11640-1-AP

  IP result of anti-CDC6(IP:11640-1-AP, 4ug; Detection:11640-1-AP 1:1000) with HeLa cells lysate 1360 ug.

• 

  IHC staining of mouse brain using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11640-1-AP (CDC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11640-1-AP (CDC6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 11640-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11640-1-AP (CDC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11640-1-AP

  Immunofluorescent analysis of HepG2 cells, using CDC6 antibody 11640-1-AP at 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, SMMC-7721-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

11640-1-AP bindet in WB, IP, IHC, IF, ELISA CDC6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDC6 fusion protein Ag2228
• Vollständiger Name: cell division cycle 6 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 560 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC025232
• Gene symbol: CDC6
• Gene ID (NCBI): 990
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. The active Cdc6 functioning as a dimer at replication origins (10793143,15096526). It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CDC6.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; A novel LncRNA transcript, RBAT1, accelerates tumorigenesis through interacting with HNRNPL and cis-activating E2F3.; Authors - Xiaoyu He
human	IF	Proc Natl Acad Sci U S A; Cell division cycle 6, a mitotic substrate of polo-like kinase 1, regulates chromosomal segregation mediated by cyclin-dependent kinase 1 and separase.; Authors - Yim Hyungshin H
mouse	IF	PLoS Genet; Histone deacetylase 2 (HDAC2) regulates chromosome segregation and kinetochore function via H4K16 deacetylation during oocyte maturation in mouse.; Authors - Ma Pengpeng P
human	WB	Int J Mol Sci; Norcantharidin Induces Immunogenic Cell Death of Bladder Cancer Cells through Promoting Autophagy in Acidic Culture.; Authors - Lili Xu
human	WB	Aging (Albany NY); β-Elemene enhances radiosensitivity in non-small-cell lung cancer by inhibiting epithelial-mesenchymal transition and cancer stem cell traits via Prx-1/NF-kB/iNOS signaling pathway.; Authors - Kun Zou
human	WB,IHC	Eur J Cancer; Y-box binding protein-1 (YB-1) promotes cell cycle progression through CDC6-dependent pathway in human cancer cells.; Authors - Basaki Yuji Y ; KD Validated