Cadherin-17 Polyklonaler Antikörper

Cadherin-17 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 24339-1-AP

Synonyme

CDH17, Intestinal peptide-associated transporter HPT-1, HPT1, HPT 1, CDH16

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  WB analysis using 24339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24339-1-AP (Cadherin-17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 24339-1-AP (Cadherin-17 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 24339-1-AP (Cadherin-17 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24339-1-AP (Cadherin-17 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24339-1-AP (Cadherin-17 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse colon using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24339-1-AP (Cadherin-17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24339-1-AP (Cadherin-17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24339-1-AP (Cadherin-17 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 24339-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24339-1-AP (Cadherin-17 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Magenkrebsgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

24339-1-AP bindet in WB, IHC, IF, IP, ELISA Cadherin-17 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cadherin-17 fusion protein Ag19488
• Vollständiger Name: cadherin 17, LI cadherin (liver-intestine)
• Berechnetes Molekulargewicht: 832 aa, 92 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC113464
• Gene symbol: Cadherin-17
• Gene ID (NCBI): 1015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.

Publikationen

Species	Application	Title
human	WB	Elife; HNF1A is a novel oncogene that regulates human pancreatic cancer stem cell properties.; Authors - Ethan V Abel
human	IHC	Jpn J Clin Oncol; Establishing metastatic patient-derived xenograft model for colorectal cancer.; Authors - Yanmei Zhang
human	WB	J Biol Chem; Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysis; Authors - Norihiro Kotani
human	WB	Int J Mol Sci; A Regulator Role for the ATP-Binding Cassette Subfamily C Member 6 Transporter in HepG2 Cells: Effect on the Dynamics of Cell-Cell and Cell-Matrix Interactions; Authors - Ilenia Matera
human	WB,IHC,IF,IP	J Exp Clin Cancer Res; A complex of cadherin 17 with desmocollin 1 and p120-catenin regulates colorectal cancer migration and invasion according to the cell phenotype; Authors - Rubén A Bartolomé ; KD Validated