CDH26 Rekombinanter Antikörper

CDH26 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240934F9

Kat-Nr. : 84143-1-RR

Synonyme

Cadherin-like protein VR20, Cadherin-like protein 26, 240934F9

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  WB analysis using 84143-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84143-1-RR (CDH26 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of U-2 OS using 84143-1-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug CDH26 Recombinant antibody (84143-1-RR, Clone:240934F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 84143-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDH26 Recombinant antibody (84143-1-RR, Clone:240934F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, MCF-7-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in FC (Intra): U-2 OS, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84143-1-RR bindet in WB, FC (Intra), ELISA CDH26 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cadherin-like 26
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: NM_021810
• Gene symbol: CDH26
• Gene ID (NCBI): 60437
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDH26, also named VR20, is a member of cadherins which are calcium-dependent cell adhesion proteins. Cadherins preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. CDH26 produces 4 isoforms through alternative splicing, with molecular weights of 13, 18, 92, and 95 kDa, respectively.