CDR2L Polyklonaler Antikörper

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• KD/KO Validated

CDR2L Polyklonal Antikörper für IF, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14563-1-AP

Synonyme

CDR2L, HUMPPA, Paraneoplastic 62 kDa antigen

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 14563-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14563-1-AP (CDR2L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14563-1-AP

  WB result of CDR2L antibody (14563-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDR2L transfected SH-SY5Y cells.

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  IP experiment of SH-SY5Y using 14563-1-AP

  IP Result of anti-CDR2L (IP:14563-1-AP, 3ug; Detection:14563-1-AP 1:500) with SH-SY5Y cells lysate 2500ug.

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  IHC staining of human cerebellum using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14563-1-AP

  Immunofluorescent analysis of HepG2 cells using 14563-1-AP (CDR2L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Cerebellum-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

14563-1-AP bindet in WB, IP, IHC, IF, ELISA CDR2L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDR2L fusion protein Ag6127
• Vollständiger Name: cerebellar degeneration-related protein 2-like
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC047534
• Gene symbol: CDR2L
• Gene ID (NCBI): 30850
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm. The predicted MW of this protein is 53 kDa.

Publikationen

Species	Application	Title
human	IP, WB	Acta Neuropathol; Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis.; Authors - Manja Schubert
human	WB,IHC	Cancer Immunol Immunother; Cerebellar degeneration-related proteins 2 and 2-like are present in ovarian cancer in patients with and without Yo antibodies.; Authors - Margrethe Raspotnig
human	WB,IF,IP	Ann Clin Transl Neurol; Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration.; Authors - Ida Herdlevaer
human,rat	WB,IHC	J Neuroimmunol; Expression of cerebellar degeneration-related proteins CDR2 and CDR2L in human and rat brain tissue.; Authors - Margrethe Raspotnig