CEBPB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CEBPB Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, RIP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Kat-Nr. : 23431-1-AP

Synonyme

C/EBP beta, CEBPB, CRP2, IL6DBP, LAP, Liver activator protein, NF IL6, Nuclear factor NF IL6, TCF 5, TCF5, Transcription factor 5

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Galerie der Validierungsdaten

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  WB analysis using 23431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23431-1-AP (CEBPB antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 23431-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23431-1-AP (CEBPB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 23431-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CEBPB antibody (23431-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IF: See 2 publications below
• CoIP: See 3 publications below
• ChIP: See 5 publications below
• RIP: See 1 publications below

Produktinformation

23431-1-AP bindet in WB, RIP, IHC, IF, CoIP, ChIP, ELISA CEBPB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEBPB fusion protein Ag20073
• Vollständiger Name: CCAAT/enhancer binding protein (C/EBP), beta
• Berechnetes Molekulargewicht: 345 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC007538
• Gene symbol: CEBPB
• Gene ID (NCBI): 1051
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298). This antibody is specific to CEBPB.

Publikationen

Species	Application	Title
mouse,rat	CoIP,WB	Adv Sci (Weinh); HIPK1 Inhibition Protects against Pathological Cardiac Hypertrophy by Inhibiting the CREB-C/EBPβ Axis; Authors - Yihua Bei
human	WB,CoIP	J Immunother Cancer; YY1 complex in M2 macrophage promotes prostate cancer progression by upregulating IL-6; Authors - Saisai Chen
human	WB	Aging (Albany NY); Functional rare variant in a C/EBP beta binding site in NINJ2 gene increases the risk of coronary artery disease.; Authors - Pengyun Wang ; KD Validated
mouse	WB	Am J Physiol Cell Physiol; The paracrine isthmin1 transcriptionally regulated by C/EBPβ exacerbates pulmonary vascular leakage in murine sepsis; Authors - Junxia Li
		Sci Rep; Identification of a 5-Methylcytosine Site that may Regulate C/EBPβ Binding and Determine Tissue-Specific Expression of the BPI Gene in Piglets.; Authors - Li Sun
mouse	WB	Ecotoxicol Environ Saf; Effect of Notch pathway on lipid accumulation induced by mono-2-ethylhexyl phthalate on 3T3-L1 cells.; Authors - Wen Qi