CELSR2 Rekombinanter Antikörper

CELSR2 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230319H5

Kat-Nr. : 83115-1-RR

Synonyme

230319H5, Cadherin EGF LAG seven-pass G-type receptor 2, Cadherin family member 10, CDHF10, EGF like protein 2

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  IF Staining of SH-SY5Y using 83115-1-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CELSR2 antibody (83115-1-RR, Clone: 230319H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 83115-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CELSR2 Recombinant antibody (83115-1-RR, Clone:230319H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83115-1-RR bindet in IF/ICC, FC (Intra), ELISA CELSR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
• Berechnetes Molekulargewicht: 318 kDa
• GenBank-Zugangsnummer: NM_001408
• Gene symbol: CELSR2
• Gene ID (NCBI): 1952
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CELSR2, also named CDHF10, EGFL2, KIAA0279, and MEGF3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is a receptor that may be important in cell/cell signaling during nervous system formation.