CEP120 Polyklonaler Antikörper

CEP120 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24067-1-AP

Synonyme

CCDC100, centrosomal protein 120kDa, Centrosomal protein of 120 kDa, CEP120

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 24067-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24067-1-AP (CEP120 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24067-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24067-1-AP (CEP120 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 24067-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24067-1-AP (CEP120 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 24067-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24067-1-AP (CEP120 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24067-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP120 antibody (24067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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  IF Staining of HeLa using 24067-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP120 antibody (24067-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24067-1-AP bindet in WB, IHC, IF, ELISA CEP120 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP120 fusion protein Ag21165
• Vollständiger Name: centrosomal protein 120kDa
• Berechnetes Molekulargewicht: 986 aa, 113 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC040527
• Gene symbol: CEP120
• Gene ID (NCBI): 153241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP120 encodes a protein that functions in the microtubule-dependent coupling of the nucleus and the centrosome. It is involved in regulating centrosome-mediated interkinetic nuclear migration of neural progenitors (PubMed: 27185865, PubMed: 23857771). CEP120, SPICE1 and CPAP are required for centriole elongation and for recruitment of distal microtubule-capping proteins and CEP135 to procentrioles (PubMed: 23810536). Cep120 and Taccs are required for the neural progenitor pool during mouse neocortical development (PubMed: 17920017).