CEP135 Polyklonaler Antikörper

CEP135 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (1)

Kat-Nr. : 24428-1-AP

Synonyme

centrosomal protein 135kDa, Centrosomal protein 4, Centrosomal protein of 135 kDa, CEP135, CEP4, KIAA0635

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  WB analysis using 24428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24428-1-AP (CEP135 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24428-1-AP (CEP135 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MDCK using 24428-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 24428-1-AP (CEP135 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 24428-1-AP

  Immunofluorescent analysis of MDCK cells using 24428-1-AP (CEP135 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, U2OS-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 8 publications below

Produktinformation

24428-1-AP bindet in WB, IF/ICC, ELISA CEP135 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP135 fusion protein Ag19122
• Vollständiger Name: centrosomal protein 135kDa
• Berechnetes Molekulargewicht: 1140 aa, 134 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC136535
• Gene symbol: CEP135
• Gene ID (NCBI): 9662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.

Publikationen

Species	Application	Title
human	WB	Curr Biol; SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.; Authors - Hindol Gupta
	WB	Cell Rep; DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling; Authors - Yu-Qian Mao
mouse	IF	EMBO Rep; ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.; Authors - Ting Song
human		Elife; WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.; Authors - Emmanuelle Steib
human	IF	Cells; Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells.; Authors - Zhenzhen Chu
human	IF	J Cell Sci; Separation-of-function MCPH-associated mutations in CPAP affect centriole number and length; Authors - Sonal Jaiswal

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (01-25-2023)

RPE1 cells stained for Hoechst (DNA marker, in blue), Cep135 (in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

CEP135 Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:24428-1-AP)