CEP170/CEP170L Polyklonaler Antikörper

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• KD/KO Validated

CEP170/CEP170L Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 18899-1-AP

Synonyme

CEP170, CEP170/CEP170L, CEP170L, FAM68B, KIAA0470, KIAA0470L

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Galerie der Validierungsdaten

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  WB analysis using 18899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 18899-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 18899-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 18899-1-AP (CEP170/CEP170L antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 18899-1-AP

  Immunofluorescent analysis of Hela cells, using CEP170L antibody 18899-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

18899-1-AP bindet in WB, IP, IF, ELISA CEP170/CEP170L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP170/CEP170L fusion protein Ag13532
• Vollständiger Name: centrosomal protein 170kDa-like
• Berechnetes Molekulargewicht: 170 kDa
• Beobachtetes Molekulargewicht: 170-200 kDa
• GenBank-Zugangsnummer: BC014590
• Gene symbol: CEP170L
• Gene ID (NCBI): 645455
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP170, also named as FAM68A, KAB and KIAA0470, is a fork head-associated (FHA) domain protein. CEP170L is a part of CEP170. The gene CEP170 has some isoforms with MW 170-200 kDa, 160 kDa and some fragments 30-40 kDa, ~75 kDa.

Publikationen

Species	Application	Title
human	WB,IF,IP	Mol Cancer; CHEK1 and circCHEK1_246aa evoke chromosomal instability and induce bone lesion formation in multiple myeloma.; Authors - Chunyan Gu
human	WB,IP	Signal Transduct Target Ther; BUB1B and circBUB1B_544aa aggravate multiple myeloma malignancy through evoking chromosomal instability.; Authors - Xiaozhu Tang ; KD Validated
human	WB	Acta Pharm Sin B; NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma; Authors - Rongfang Wei