CEP250/CNAP1 Polyklonaler Antikörper

CEP250/CNAP1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Rezensionen (2)

Kat-Nr. : 14498-1-AP

Synonyme

250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CEP250/CNAP1, CNAP1, C-NAP1

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 14498-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14498-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14498-1-AP

  IP Result of anti-CEP250/CNAP1 (IP:14498-1-AP, 5ug; Detection:14498-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human placenta using 14498-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14498-1-AP (CEP250/CNAP1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 14498-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14498-1-AP (CEP250/CNAP1 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 14498-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 14498-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP250/CNAP1 antibody (14498-1-AP) at dilution of 1:100 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L), SCLT1 antibody (14875-1-AP, Magenta), CoraLite®488 ARL13B antibody (CL488-17711, green).

• 

  IF Staining of HeLa using 14498-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 30 publications below

Produktinformation

14498-1-AP bindet in WB, IP, IHC, IF, ELISA CEP250/CNAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP250/CNAP1 fusion protein Ag5925
• Vollständiger Name: centrosomal protein 250kDa
• Berechnetes Molekulargewicht: 281 kDa
• Beobachtetes Molekulargewicht: 250 kDa
• GenBank-Zugangsnummer: BC001433
• Gene symbol: CEP250
• Gene ID (NCBI): 11190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.

Publikationen

Species	Application	Title
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
human	IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
mouse	IF	Nat Commun; Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.; Authors - Munemasa Mori
human	IF	PLoS Biol; Stable centrosomal roots disentangle to allow interphase centriole independence.; Authors - Robert Mahen
human	WB,IF	Proc Natl Acad Sci U S A; Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins.; Authors - Igor Cervenka
	IF	J Cell Biol; Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals.; Authors - Kan Yaguchi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Karsten (Verified Customer) (12-22-2022)

WB: 1:600 dilution in 5% Milch in 1xTBST over nigth at 4°C, 30µg Protein loaded: result show prominent band at a hight of 250 kDa. unspecific bands also visible, but not prominent. worked fine IFS: 1:100, MetOH (-20°) fixation for 5 min at RT , Permeabilisation with 0.3% PBST (Triton) for 5 min at RT, Blocking with 1% BSA in PBS for 1h at RT. Antibody dilution 1:100 in 1%BSA in PBS 1h RT, sek Ak 1h at RT- nice basal body stainings at cilia in hTERT-RPE1 cells, that were starved for 3 days.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: hTERT-RPE1 cells, 3 days starved

CEP250/CNAP1 Antibody Western Blot,Immunofluorescence validation (1:600 dilution) in hTERT-RPE1 cells, 3 days starved (Cat no:14498-1-AP)

FH

Hairuo (Verified Customer) (12-16-2019)

Used in IF in neonatal mouse testis cry-sections.Works well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500

Verwandte Produkte

NEK2 Polyclonal antibody

14233-1-AP

CEP135 Polyclonal antibody

24428-1-AP

PPP1CC Polyclonal antibody

11082-1-AP

CDC42EP4 Polyclonal antibody

19262-1-AP

CDC42EP2 Polyclonal antibody

11824-1-AP

CDC42 Polyclonal antibody

10155-1-AP