CEP55 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CEP55 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 23891-1-AP

Synonyme

C10orf3, centrosomal protein 55kDa, Centrosomal protein of 55 kDa, Up regulated in colon cancer 6, Up-regulated in colon cancer 6

---

• 

  WB analysis of HeLa using 23891-1-AP

  WB result of CEP55 antibody (23891-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CEP55 transfected HeLa cells.

• 

  WB analysis using 23891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 23891-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 23891-1-AP

  IP result of anti-CEP55 (IP:23891-1-AP, 4ug; Detection:23891-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human testis using 23891-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23891-1-AP (CEP55 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 23891-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23891-1-AP (CEP55 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 23891-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CEP55 antibody (23891-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hodengewebe, Jurkat-Zellen, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

23891-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CEP55 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP55 fusion protein Ag20940
• Vollständiger Name: centrosomal protein 55kDa
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 47-55 kDa
• GenBank-Zugangsnummer: BC008947
• Gene symbol: CEP55
• Gene ID (NCBI): 55165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.

Publikationen

Species	Application	Title
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
human	WB,IP,IF	Cell Death Dis; iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.; Authors - Kun Gao ; KD Validated
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	Front Oncol; USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.; Authors - Catherine M Nickson ; KD Validated
human	WB,IHC	Cells; CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.; Authors - Minjing Li ; KD Validated
human	IF	iScience; Non-muscle Myosin-II Is Required for the Generation of a Constriction Site for Subsequent Abscission.; Authors - Kangji Wang