CEP68 Polyklonaler Antikörper

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• KD/KO Validated

CEP68 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 15147-1-AP

Synonyme

Centrosomal protein of 68 kDa

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  WB analysis using 15147-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15147-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HeLa-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IF: See 6 publications below

Produktinformation

15147-1-AP bindet in WB, IF, ELISA CEP68 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP68 fusion protein Ag7447
• Vollständiger Name: centrosomal protein 68kDa
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 67 kDa
• GenBank-Zugangsnummer: BC002982
• Gene symbol: CEP68
• Gene ID (NCBI): 23177
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP68, or centrosomal protein 68, is a protein-coding gene in humans. It is involved in the maintenance of centrosome cohesion, likely as part of a linker structure that prevents centrosome splitting. CEP68 is also required for the localization of CDK5RAP2 to the centrosome during interphase and contributes to the formation of CROCC/rootletin filaments. This protein is encoded by the CEP68 gene and is located on the fibers emanating from the proximal ends of centrioles. It has been associated with diseases such as Retinitis Pigmentosa 28 and is implicated in angioedema. The gene is mapped to chromosome 2 and is expressed ubiquitously in various tissues, including the brain, ovary, and others. The protein plays a crucial role in cellular functions related to the centrosome, which is a key organelle in cell division and organization. This antibody recognized two bands of ∼90 and 67 kDa, respectively, in good agreement with the predicted sizes of the two Cep68 isoforms (81 and 67 kDa for Cep68L and -S, respectively).

Publikationen

Species	Application	Title
human	WB,IF	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin; Authors - Delowar Hossain ; KD Validated
human	WB	J Virol; The Cytoskeletal Adaptor Obscurin-Like 1 Interacts with the Human Papillomavirus 16 (HPV16) Capsid Protein L2 and Is Required for HPV16 Endocytosis.; Authors - Elena Wüstenhagen ; KD Validated
human	IF	Sci Rep; Prolonged overexpression of PLK4 leads to formation of centriole rosette clusters that are connected via canonical centrosome linker proteins; Authors - Selahattin Can Ozcan
human	WB,IF	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin.; Authors - Delowar Hossain ; KD Validated
human	WB	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao
human	WB,IF	iScience; Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacement.; Authors - Elena Fdez