Complement factor D Polyklonaler Antikörper

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• KD/KO Validated

Complement factor D Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26050-1-AP

Synonyme

CFD, EC:3.4.21.46, DF, complement factor D (adipsin), C3 convertase activator

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  WB analysis of human plasma using 26050-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 26050-1-AP (Complement factor D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 26050-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 26050-1-AP (Complement factor D Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human stomach using 26050-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26050-1-AP (Complement factor D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

26050-1-AP bindet in WB, IHC, ELISA Complement factor D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Complement factor D fusion protein Ag23473
• Vollständiger Name: complement factor D (adipsin)
• Berechnetes Molekulargewicht: 253 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC057807
• Gene symbol: CFD
• Gene ID (NCBI): 1675
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complement factor D, also known as adipsin, is a highly specific serine protease. Complement factor D is the rate-limiting enzyme in the alternative pathway of complement. It activates C3 convertase by cleaving factor B, which is a key step in the alternative pathway. Complement factor D catalyzes the cleavage of factor B to generate Ba (non-catalytic chain) and Bb (active catalytic subunit), which together with C3(H2O) (or C3b) constitute the active C3 convertase C3(H2O)Bb (or C3bBb). (PMID: 34566967; PMID: 27775713).

Publikationen

Species	Application	Title
human	WB	Immunol Res; The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy.; Authors - Muyao Ye
human	WB	Hypertens Res; Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation; Authors - Zheng Wang ; KD Validated