Cofilin Polyklonaler Antikörper

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• KD/KO Validated

Cofilin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Kat-Nr. : 10960-1-AP

Synonyme

CFL1, 18 kDa phosphoprotein, Cofilin-1, Cofilin1, Cofilin, non-muscle isoform

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  WB analysis using 10960-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10960-1-AP (Cofilin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skin using 10960-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10960-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (Cofilin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10960-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10960-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10960-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10960-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10960-1-AP (Cofilin antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human cervical cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:25 (under 40x lens).

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  IHC staining of human lung cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10960-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of NIH/3T3 using 10960-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Cofilin antibody (10960-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 10960-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 10960-1-AP (Cofilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, humanes Hirngewebe, humanes Hautgewebe, Maushirngewebe, NCI-H1299-Zellen, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Zervixkarzinomgewebe, humanes Herzgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:600-1:2400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10960-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Cofilin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cofilin fusion protein Ag1399
• Vollständiger Name: cofilin 1 (non-muscle)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18-22 kDa
• GenBank-Zugangsnummer: BC012318
• Gene symbol: Cofilin
• Gene ID (NCBI): 1072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Publikationen

Species	Application	Title
	WB,IF,IP	Nat Commun; N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization; Authors - Weiji Weng
human	WB,IF	Cell Res; NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.; Authors - Cheng Zhang ; KD Validated
Human		Cell Prolif; An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms; Authors - Nan Zhang
human	WB	Front Immunol; Development of a prognostic model for hepatocellular carcinoma based on microvascular invasion characteristic genes by spatial transcriptomics sequencing; Authors - Xiaolan Mu
human	WB	Int J Mol Sci; Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks.; Authors - Marina Damato
mouse	WB	Commun Biol; RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling; Authors - Haixia Zhu