CFTR Polyklonaler Antikörper

CFTR Polyklonal Antikörper für IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 20738-1-AP

Synonyme

ABCC7, ATP-binding cassette sub-family C member 7, cAMP-dependent chloride channel, CFTR/MRP, Channel conductance-controlling ATPase

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  IHC staining of human lung using 20738-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20738-1-AP (CFTR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 20738-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20738-1-AP (CFTR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20738-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20738-1-AP (CFTR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20738-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20738-1-AP (CFTR Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 20738-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20738-1-AP (CFTR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 20738-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20738-1-AP (CFTR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HUVEC using 20738-1-AP

  1X10^6 HUVEC cells were intracellularly stained with 0.2 ug Anti-Human CFTR (20738-1-AP) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HUVEC-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

20738-1-AP bindet in WB, IHC, IF, FC (Intra), ELISA CFTR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
• Berechnetes Molekulargewicht: 168 kDa
• GenBank-Zugangsnummer: NM_000492
• Gene symbol: CFTR
• Gene ID (NCBI): 1080
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

      What is the human cytogenetic location of the CFTR gene?

7q31.2

What is the predicted molecular weight of CFTR? 

168 kDa

Where is CFTR expressed?

CFTR is mainly expressed in apical membranes of vertebrate epithelial cells.

What is the ion selectivity of CFTR?

CFTR is an anion-selective channel, responsible for regulating chloride and bicarbonate transport.

What is the process of CFTR trafficking?

The ER provides the first steps in CFTR folding quality control. CFTR can be degraded by the ER associated degradation (ERAD) pathway, or it is transported to the Golgi for maturation before being trafficked to the cell membrane.

What are the separate domains of CFTR?

CFTR consists of two transmembrane domains, two nucleotide binding domains, and a regulatory domain.

What regulates CFTR?

CFTR activity is ATP-dependent and is regulated by protein kinase A phosphorylation.

What is the role of CFTR?

CFTR functions as a channel for anions, regulating water and ionic homeostasis.

What mutations in CFTR leads to protein degradation?

Deletion of the regulatory domain or the phenylalanine 508 (F508del) results in an increase in CFTR degradation. This degradation then leads to a decrease of secretion product clearance and an increase in viscous mucosal buildup at the epithelial surface of affected organs, the primary symptom of cystic fibrosis. Mutations in CFTR can also lead congenital bilateral absence of vas deferens (CBAVD) or sperm abnormalities in men.

How does CFTR differ from other ATP-binding cassette proteins?

Unlike other proteins in the ABC family, CFTR functions as an ion channel.

PMIDs: 24513531, 24534272, 22966013, 22709980

Publikationen

Species	Application	Title
mouse	WB,IF	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
human	IF	PLoS One; Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet.; Authors - Mauricio Di Fulvio
human	WB	J Exp Clin Cancer Res; Oridonin promotes endoplasmic reticulum stress via TP53-repressed TCF4 transactivation in colorectal cancer; Authors - Fangyuan Zhou
human	IHC	Int Immunopharmacol; A novel transcriptomic signature associated with lymphovascular invasion predicts clinical outcomes, tumor microenvironment, and therapeutic response in lung adenocarcinoma; Authors - Xing Huang
mouse	IF	Biochem Biophys Res Commun; Dapagliflozin administration to a mouse model of type 2 diabetes induces DNA methylation and gene expression changes in pancreatic islets; Authors - Aisha Yokoi
human	IF	Asian J Androl; The role of SLC26A8 homozygous variants in male infertility and flagellum abnormalities; Authors - Bin Mao