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CHAD Polyklonaler Antikörper

CHAD Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12963-1-AP

Synonyme

Cartilage leucine rich protein, CHAD, chondroadherin, SLRR4A

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HeLa cells using CHAD Polyclonal antibody (12963-1-AP)

    WB analysis of HeLa using 12963-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CHAD Polyclonal antibody (12963-1-AP)

    WB analysis of MCF-7 using 12963-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CHAD Polyclonal antibody (12963-1-AP)

    WB analysis of Jurkat using 12963-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

12963-1-AP bindet in WB,ELISA CHAD und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CHAD fusion protein Ag4077
Vollständiger Name chondroadherin
Berechnetes Molekulargewicht 359 aa, 40.5 kDa
Beobachtetes Molekulargewicht 41 kDa
GenBank-ZugangsnummerBC036360
Gene symbol CHAD
Gene ID (NCBI) 1101
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chondroadherin (CHAD), also known as cartilage leucine-rich protein, is a prominent noncollagenous extracellular protein in cartilage. It was first isolated from bovine cartilage. The primary structure of CHAD shows that it belongs to the family of leucine-rich repeat (LRR) proteins. It is expressed at high levels in certain zones of cartilage and also has been detected in bone, tendon, bone marrow, and chondrosarcoma cells. CHAD promotes attachment of chondrocytes, fibroblasts, and osteoblasts, mediated via the integrin alpha2beta1. It has also been shown to bind to collagen type II and both collagen type II and chondroadherin interact with integrin alpha2beta1. Besides, CHAD may play an important role in the regulation of chondrocyte growth and proliferation.

Protokolle

Produktspezifische Protokolle
WB protocol for CHAD antibody 12963-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Reprod Sci

NFAT Overexpression Correlates with CA72-4 and Poor Prognosis of Ovarian Clear-Cell Carcinoma Subtype.

Authors - Bing Xin
View Article