CHAF1B Polyklonaler Antikörper

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• KD/KO Validated

CHAF1B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27633-1-AP

Synonyme

MPP 7, p60 CAF1, CAF1, CAF IP60, CAF I p60

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  WB analysis using 27633-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 27633-1-AP (CHAF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27633-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27633-1-AP (CHAF1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27633-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27633-1-AP (CHAF1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27633-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27633-1-AP (CHAF1B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 27633-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27633-1-AP (CHAF1B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

27633-1-AP bindet in WB, IHC, ChIP, ELISA CHAF1B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHAF1B fusion protein Ag26504
• Vollständiger Name: chromatin assembly factor 1, subunit B (p60)
• Berechnetes Molekulargewicht: 559 aa, 61 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC021218
• Gene symbol: CHAF1B
• Gene ID (NCBI): 8208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHAF1B, also named as CAF1A, CAF1P60, MPHOSPH7, MPP7, is a 559 amino acid protein, which belongs to the WD repeat HIR1 family. CHAF1B as a subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1B is thought to mediate chromatin assembly in DNA replication and DNA repair and assembles histone octamers onto replicating DNA in vitro. The calcualted molecular weight of CHAF1B is 61 kDa, but molecular weight of CHAF1B is detected about 65-70 kDa.

Publikationen

Species	Application	Title
human	WB	Biomed Pharmacother; Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.; Authors - Shuai Huang ; KD Validated
human	ChIP	Cancer Res Commun; Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells; Authors - Diana Saleiro