CHCHD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CHCHD2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(43)

Kat-Nr. : 19424-1-AP

Synonyme

C7orf17, CHCHD2, MNRR1, NS2TP

---

• 

  WB analysis using 19424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19424-1-AP

  WB result of CHCHD2 antibody (19424-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells.

• 

  WB analysis using 19424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 19424-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 19424-1-AP

  IP result of anti-CHCHD2 (IP:19424-1-AP, 4ug; Detection:19424-1-AP 1:1000) with HepG2 cells lysate 2520 ug.

• 

  IHC staining of human lung cancer using 19424-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19424-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19424-1-AP (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 19424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19424-1-AP (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 19424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (19424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 19424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (19424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 19424-1-AP

  Immunofluorescent analysis of HepG2 cells, using CHCHD2 antibody 19424-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Nebennierengewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 38 publications below
• IHC: See 3 publications below
• IF: See 9 publications below
• CoIP: See 2 publications below

Produktinformation

19424-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA CHCHD2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, drosophila, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHCHD2 fusion protein Ag13752
• Vollständiger Name: coiled-coil-helix-coiled-coil-helix domain containing 2
• Berechnetes Molekulargewicht: 151 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16-18 kDa
• GenBank-Zugangsnummer: BC003079
• Gene symbol: CHCHD2
• Gene ID (NCBI): 51142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).

Publikationen

Species	Application	Title
human	WB, IF	Lancet Neurol; CHCHD2 mutations in autosomal dominant late-onset Parkinson's disease: a genome-wide linkage and sequencing study.; Authors - Manabu Funayama
Drosophila	WB	Nat Commun; Loss of Parkinson's disease-associated protein CHCHD2 affects mitochondrial crista structure and destabilizes cytochrome c.; Authors - Hongrui Meng
human	WB	Nat Commun; TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro.; Authors - Minwoo Baek
	WB	J Biomed Sci; Genetic and pharmacologic p32-inhibition rescue CHCHD2-linked Parkinson's disease phenotypes in vivo and in cell models; Authors - Murni Tio
human	WB	Proc Natl Acad Sci U S A; Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) rescues the cellular phenotype of MELAS by inducing homeostatic mechanisms.; Authors - Siddhesh Aras
human	WB	Cell Death Dis; CHCHD2 rescues the mitochondrial dysfunction in iPSC-derived neurons from patient with Mohr-Tranebjaerg syndrome; Authors - Yihua Huang