CHD3 Polyklonaler Antikörper

CHD3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25335-1-AP

Synonyme

hZFH, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 3, CHD-3, CHD 3

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  WB analysis using 25335-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25335-1-AP (CHD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25335-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25335-1-AP (CHD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25335-1-AP bindet in WB, IHC, ELISA CHD3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: chromodomain helicase DNA binding protein 3
• Berechnetes Molekulargewicht: 227 kDa
• Beobachtetes Molekulargewicht: 240 kDa
• GenBank-Zugangsnummer: NM_005852
• Gene symbol: CHD3
• Gene ID (NCBI): 1107
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development; Authors - Xue Chen