CHDH Polyklonaler Antikörper

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• KD/KO Validated

CHDH Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 17356-1-AP

Synonyme

CDH, CHD, CHDH, Choline dehydrogenase

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Galerie der Validierungsdaten

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  WB analysis using 17356-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17356-1-AP (CHDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17356-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17356-1-AP (CHDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17356-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17356-1-AP (CHDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17356-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17356-1-AP (CHDH antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17356-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17356-1-AP (CHDH antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, Mauslebergewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

17356-1-AP bindet in WB, IHC, IF, ELISA CHDH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHDH fusion protein Ag10614
• Vollständiger Name: choline dehydrogenase
• Berechnetes Molekulargewicht: 594 aa, 65 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC034502
• Gene symbol: CHDH
• Gene ID (NCBI): 55349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHDH(Choline dehydrogenase, mitochondrial) is also named as CDH,CHD and belongs to the GMC oxidoreductase family. CHDH is a member of the glucose-methanol-choline family of oxidoreductases, with each member containing a consensus FAD-binding domain. Many members of this oxidoreductase family are soluble proteins but mammalian CHDH is associated with the inner mitochondrial membrane(Handbook of vitamins,Robert B,2007). The full length protein(65 kDa) has a transit peptide of 29 amino acid and the mature CHDH is appropriately located in the inner mitochondria membrane(PMID:12951056). 17356-1-AP can also recognize the 45 kDa isoform X3 of CHDH.

Publikationen

Species	Application	Title
mouse	WB	J Biol Chem; Betaine is accumulated via transient choline dehydrogenase activation during Mouse oocyte meiotic maturation.; Authors - Taylor McClatchie ; KO Validated
human	IHC	PLoS One; Choline dehydrogenase polymorphism rs12676 is a functional variation and is associated with changes in human sperm cell function.; Authors - Johnson Amy R AR
human	IHC	Transl Oncol; Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level.; Authors - Xuyong Wei
human	IF	Sci Adv; Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression; Authors - Hao Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wojciech (Verified Customer) (03-23-2023)

A band was detected at around 60 kDa. 30 ug of protein was loaded and probed with the antibody at 1:2000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: T-ALL cell line (CCRF-CEM)

FH

Hua (Verified Customer) (12-04-2018)

40 ug of mouse liver lysate was loaded onto the gel. The incubation time is over night at 4oC.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver

CHDH Antibody Western Blot, validation (1:1000 dilution) in mouse liver (Cat no:17356-1-AP)