CHFR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CHFR Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 12169-1-AP

Synonyme

CHFR, RING finger protein 196, RNF116, RNF196

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 12169-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells/tissue using 12169-1-AP

  WB result of 12169-1-AP (CHFR antibody).

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  WB analysis of Jurkat using 12169-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12169-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 12169-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12169-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human endometrial cancer using 12169-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12169-1-AP (CHFR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 12169-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12169-1-AP (CHFR antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 12169-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12169-1-AP (CHFR antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Multizellen/-gewebe
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

12169-1-AP bindet in WB, IHC, ELISA CHFR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHFR fusion protein Ag2811
• Vollständiger Name: checkpoint with forkhead and ring finger domains
• Berechnetes Molekulargewicht: 664 aa, 73 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: BC012072
• Gene symbol: CHFR
• Gene ID (NCBI): 55743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Checkpoint with forkhead and RING finger domains protein(CHFR) is a checkpoint protein that reportedly initiates a cell cycle delay in response to microtubule stress during prophase in mitosis and it has been hypothesized to be a tumor suppressor with a potential role as a biomarker for chemotherapeutic response to Taxol(PMID:17596595). It belongs to the CHFR family. Endogenous Chfr is ubiquitinated and phosphorylated in an MPF-dependent manner in mitotic Xenopus egg extracts(PMID:18504434). It has 5 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	IHC	Clin Cancer Res; Association of CHFR promoter methylation with disease recurrence in locally advanced colon cancer.; Authors - Tanaka Motofumi M
human	WB	Transl Oncol; Silencing of CHFR Sensitizes Gastric Carcinoma to PARP Inhibitor Treatment.; Authors - Yuan Li
human	IHC	J Biol Chem; CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.; Authors - Kashima Lisa L
human	WB	Front Genet; Identification of a Novel Epigenetic Signature CHFR as a Potential Prognostic Gene Involved in Metastatic Clear Cell Renal Cell Carcinoma.; Authors - Xiangling Chen ; KD Validated
human	WB	Mol Med Rep; CHFR‑mediated epithelial‑to‑mesenchymal transition promotes metastasis in human breast cancer cells.; Authors - Guobin Jiang ; KD Validated
human,mouse	WB	Nat Commun; Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions; Authors - Chinnaswamy Tiruppathi ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (02-11-2022)

This antibody works for me great

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:500 for IF
• Cell Tissue Type: Colon cancer cells