Chromogranin A Polyklonaler Antikörper

Chromogranin A Polyklonal Antikörper für IHC, IF/ICC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 23342-1-AP

Synonyme

CGA, CHGA, AL-11, Chromogranin-A, EA-92

---

• 

  IHC staining of human lung cancer using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 10x lens).

• 

  IHC staining of human small intestine using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human appendicitis using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 10x lens).

• 

  IHC staining of human colon using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 23342-1-AP (Chromogranin A antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of mouse pancreas using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 23342-1-AP (Chromogranin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23342-1-AP (Chromogranin A antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of mouse colon using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23342-1-AP (Chromogranin A antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human lung cancer using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 23342-1-AP (Chromogranin A antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 23342-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 23342-1-AP (Chromogranin A antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse small intestine using 23342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (23342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 23342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (23342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 23342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using Chromogranin A antibody (23342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of Neuro-2a using 23342-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using Chromogranin A antibody (23342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe, humanes Appendizitis-Gewebe, humanes Kolongewebe, humanes Lungenkarzinomgewebe, humanes Dünndarmgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF/ICC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

23342-1-AP bindet in IHC, IF/ICC, IF-P, IF-Fro, ELISA Chromogranin A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Chromogranin A fusion protein Ag19780
• Vollständiger Name: chromogranin A (parathyroid secretory protein 1)
• Berechnetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC006459
• Gene symbol: Chromogranin A
• Gene ID (NCBI): 1113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Publikationen

Species	Application	Title
		EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
human	IHC	Front Immunol; New insights into markers for distinguishing neuroendocrine prostate cancer: evidence from single-cell analysis; Authors - Hailang Luo
mouse	IF	Cell Mol Gastroenterol Hepatol; Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia; Authors - Pawan Puri