Choline Kinase Alpha Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Choline Kinase Alpha Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (1)

Kat-Nr. : 13520-1-AP

Synonyme

CHETK alpha, CHK, CHKA, Choline kinase alpha, CK, CKI, CK-α, EK, Ethanolamine kinase;

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Galerie der Validierungsdaten

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  WB analysis using 13520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13520-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 13520-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 13520-1-AP

  IP Result of anti-Choline kinase alpha (IP:13520-1-AP, 4ug; Detection:13520-1-AP 1:500) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human liver cancer using 13520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 13520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 13520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13520-1-AP (Choline kinase alpha antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13520-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13520-1-AP (Choline kinase alpha antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, COLO 320-Zellen, Maus-Kolongewebe, Rattenmilzgewebe
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• ELISA: See 1 publications below

Produktinformation

13520-1-AP bindet in WB, IP, IHC, IF, ELISA Choline Kinase Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Choline Kinase Alpha fusion protein Ag4761
• Vollständiger Name: choline kinase alpha
• Berechnetes Molekulargewicht: 439 aa, 52 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC036471
• Gene symbol: CHKA
• Gene ID (NCBI): 1119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHKA(Choline kinase alpha) is also named as CHK, CKI, EK(Ethanolamine kinase) and belongs to the choline/ethanolamine kinase family. Choline kinase (ATP:choline phosphotransferase) is a cytosolic enzyme that catalyzes the committed step in the synthesis of PC by the CDP-choline pathway. The enzyme catalyzes the phosphorylation of choline with ATP to form phosphocholine and ADP(PMID:9506987). It has 2 isoforms produced by alternative splicing with the molecular weight of 52 kDa and 50 kDa.

Publikationen

Species	Application	Title
human	WB,IHC	Proc Natl Acad Sci U S A; The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation; Authors - Qingxia Ma
mouse	WB, IHC	Ann Rheum Dis; Choline kinase inhibition in rheumatoid arthritis.; Authors - M Guma
human	WB	Clin Cancer Res; Preclinical development of a non-toxic oral formulation of monoethanolamine, a lipid precursor, for prostate cancer treatment.; Authors - Roopali Saxena
mouse	WB	Front Cell Dev Biol; Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration.; Authors - Ming Kong
rat	WB	J Biol Chem; ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.; Authors - Arsenault Daniel J DJ
	WB	Mol Biol Cell; Nuclear-localized CTP:phosphocholine cytidylyltransferase α regulates phosphatidylcholine synthesis required for lipid droplet biogenesis.; Authors - Adam J Aitchison

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (02-02-2021)

I was surprised it did not work as shown in the datasheet. It is possible jus this batch?

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse liver and mouse spleen

Choline Kinase Alpha Antibody Western Blot validation (1:500 dilution) in mouse liver and mouse spleen (Cat no:13520-1-AP)